The measurement range extends from 1 x 10e4 to 1 x 10e7 cellsml the optimal range is 1 x 10e5 to 4 x 10e6 cellsml, which is broader than that of a. Invitrogen countess cell counting chamber slides fisher. We have an invitrogen countess counter which works really well for most mammalian cell lines. Cell counter thermo scientific countess ii with counting time of 10 seconds, for cell concentrations of 10,000 to 10,000,000 cellsml, cell diameters of 7 to 60um, 5mp image resolution, pdf report format, stores 10 protocols, with touch screen and usb port, no thermal printer, not suitable for fluorescence. Count the cells using invitrogen countess automated counter. Mar 12, 2020 countess ii fl automated cell counter description with the option for a reusable slide and fluorescence capabilitiesbrightfield and two userchangeable fluorescence channelsthe invitrogen countess ii fl automated cell counter can count cells, monitor fluorescent protein expression, and measure cell viability. For safety and biohazard guidelines, refer to the safety appendix in the countess ii automated cell counter user guide pub. The countess automated cell counter is a benchtop counter designed to measure cell count and viability live, dead, and total cells accurately and precisely, using the standard trypan blue technique. Cells were seeded between 4x104 to 6x104 cellscm2 for each. The reuseable slide option for this cell counter means cutting down on consumables cost significantly. Countess ii automated cell counter fisher scientific.
The countess has a touch screen display and allows data to be transferred via usb drive to a computer for data access and storage. Invitrogen was founded in 1987 by lyle turner, joe fernandez, and william mcconnell and was incorporated in 1989. Cell counter thermo scientific countess ii fl with counting time of 10 seconds, for cell concentrations of 10,000 to 10,000,000 cellsml, cell diameters of 7 to 60um, 5mp image resolution, pdf report format, stores 10 protocols, with touch screen and usb port, no thermal printer, suitable for fluorescence. Countess ii and countess ii fl automated cell counters quick reference card qrc. More than 4,800 employees work for invitrogen and we conduct business in over 70 countries. Your updated countess ii or countes ii fl instrument will have these added benefits. It can perform cell counts as well as viability and cell calculations.
The cell counting algorithm then uses the image acquired from the best focal plane to identify cells and exclude debris, thereby calculating the total cell count. The thermo countess ii fl cell counter brings you reliable cell counting and viability assessment with the added convenience of reusable hemocytometers and the option to add fluorescence capabilities. Countess ii fl automated cell counter the countess ii fl automated cell counter is a fully automated, 3channel cell counter and assay platform that uses evos light cube technology, stateoftheart optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samplesin suspension. Thermo scientific invitrogen countess ii fl user manual pdf. Countess ii automated cell counter thermo fisher scientific. The countess ii fl automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. Having a cell counter means eliminating the subjectivity and time sink of manually counting. Invitrogen countess automated cell counter model c10281, sn.
The guides listed below are available with the countess ii or the countess ii fl automated cell counter. The countess automated cell counter offers an intuitive user interface, and provides the option to save and print cell count data using the countess software download from vitrogen. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer. Cell culture cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells e. With threechannel flexibilitybrightfield and two optional fluorescence channelsresearchers can count cells, monitor fluorescent protein. Invitrogen countess automated cell counter model c10281, sn 12121069. Invitogen cell countess nov252009 we just got the new cell countess from invitrogen and was wondering if any one else is using this equipment for cells shldbcrzy. Thermo scientific invitrogen countess ii fl manuals. Allow at least 5 cm 2 in free space at the back of the instrument to allow for. User manual thermo scientific invitrogen countess ii fl user manual 82 pages. Countess ii automated cell counters we offer two high performance automated cell counters designed to meet the needs of any lab. Invitrogen countess automated cell counter model c10281, s. The countess is selfcontained so you do not need an additional.
At the heart of the countess systems fluorescence technology lie the unique invitrogen evos light cubes. In vitro intestinal epithelial woundhealing assays using. Invitrogen countess cell counting chamber slides print share invitrogen countess cell counting chamber slides. The thermo countess ii fl cell counter also has two optional user changeable fluorescence channels to fit your needs, and comes standard with a brightfield to monitor protein expression and measure cell viability before downstream analysis. Each cube contains an led, illuminating optics, and filters. Discounted pricing has been negotiated for all orders placed through the scms system and and free shipping to those shipped directly to genelab. Tc20 automated cell counter life science research biorad. The contents of the countess automated cell counter are listed below. Read the safety data sheets sdss and follow the handling instructions.
Invitrogen countess ii fl automated cell counter from. Invitrogen countess test beads life sciences, cell analysis. Tools to accelerate your life science research thermo scientific, applied biosystems. Remove instructions for countess ii, update ui for the new sw version, rebrand. The countess from invitrogen uses slides that require loading 10 ul in each side 20 ul for the whole slide of cell solution. Apr 19, 2016 in this video, we cover all the necessary steps to set up and operate the cell counter model r1. For use at room temperature under normal laboratory conditions. A wide variety of prokaryotic cell can be counted within a very short time, usually less than a minute per sample. Countess ii fl automated cell counter from thermo fisher. Countess ii and countess ii fl automated cell counters user guide. Summary of contents for thermo scientific invitrogen countess ii fl page 1 countess ii fl automated cell counter user guide for fluorescence and brightfield applications catalog number amqaf document part number mp10826 publication number man0010644 revision e.
Insert the countess ii usb drive or equivalent into an available usb port on the instrument. Invitrogen countess cell counting chamber slides quantity. Laboratory%20instrumentsfiles0815co016471fourpillarsofachievingaccurateandprecisecellcounts. Invitrogen may be used in lieu of a light microscope hemacytometer for cell counting and viability calculation. The invitrogen countess ii automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts in as little as 10 seconds. I have been using countess ii for cancerous cell hepg2 count but never tried microalgae. Countess ii and countess ii fl automated cell counters. Mdbk left and vero right cell proliferation results in three different lots of equafetal. Invitrogen countess automated cell counter manual st. Mdbk cells were cultured in the presence of 10% fetal bovine serum and 10% equafetal in dmem with lglutamine for three passages. Slides and glass countess ii fl reusable slides on. With its unique software algorithm and innovative liquid lens technology, the model r1 is designed.
The countess automated cell counter from invitrogen is a benchtop automated cell counter. Used in cell culture applications atlas biologicals. Cell counting market size, share, trends global industry. I count them anyways and proceed to validate my results with the tc20 automated cell counter by biorad and confirm that they show similaridentical percentage of live vs dead cells. Countess ii automated cell counter catalog number amqax pub. The tc20 automated cell counter uses microscopy with autofocus that analyzes multiple focal planes to identify the best plane. We provide products and services to pharmaceutical and biotechnology companies, as well as academic and government research institutions. The invitrogen portion of the genelab supply center will be offered through a supply center management system scms hosted directly at invitrogens web server. Countess ii fl automated cell counter fisher scientific. Dilution calculatornew builtin dilution calculator displays volume of cell sample and buffer needed figure 1. But nothing counts like countess ii automated cell counters. The countess ii automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. In this video, we cover all the necessary steps to set up and operate the cell counter model r1.
On the results screen, press save to go to the save screen. Invitrogen catalog first page, datasheet, datasheet search, data sheet, datasheets, datasheet search site for electronic components and semiconductors, integrated circuits, diodes, triacs, semiconductors. Countess ii fl automated cell counter thermo fisher scientific. Countess automated cell counter university imaging centers. Each slide contains two cell counting chambers 75 mm. Countess ii automated cell counters thermo fisher scientific. The invitrogen countess automated cell counter uses trypan blue stain to stain cells for counting and viability studies. Successful cell culture depends heavily on keeping the cells free from contamination. Simplified workfloweasily save protocols from the adjustments screen. The countess automated cell counter offers easy and accurate cell counting and viability counts. Designed for use with countess automated cell counter. Press the file name text field and use the alphanumeric keypad to name your files. Select the desired files to save your experiment result, images, data.
Has anyone had experience using the thermofischer scientific. Invitrogen countess ii automated cell counter fisher. I count them anyways and proceed to validate my results with the tc20 automated cell counter by biorad and confirm that they show similaridentical percentage of. Countess automated cell counter from thermo scientific. We have 1 thermo scientific invitrogen countess ii fl manual available for free pdf download. Download the latest countess iiii fl software version to your desktop. The company initially found success with its kits for molecular cloning notably, the librarian, a kit for making cdna libraries, and the fasttrack kit for mrna isolation from biological samples.
Invitrogen countess test beads for verifying proper functioning of countess automated cell counter manufacturer. Used invitrogen countess c10227 cell counter for sale. Reportsave a printerfriendly pdf report of the results, graphs, and image figure 2. Invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. Rise in prevalence of chronic diseases that require nextgeneration therapeutics is the high impact rendering driver. May 26, 2009 the countess automated cell counter from invitrogen is a benchtop automated cell counter. The invitrogen countess ii and countess ii fl automated cell counters contain advanced autofocusing and counting algorithms to allow you to quickly and accurately identify and count cells within a population while avoiding user variation associated with manual focusing mechanisms. Invitrogen countess ii fl automated cell counter, and. Countess ii automated cell counter quick reference card 1 each the countess ii automated cell counter is a fully automated cell counter and assay platform that uses stateoftheart optics and image analysis algorithms to analyze trypan bluestained cells in suspension. For detailed instructions on using the countess automated cell counter, refer to the manual supplied with the counter or download the manual from. Light cubes are userinterchangeable and autodetected by the countess ii fl system for plugandplay capability. Sep 05, 2019 count the cells using invitrogen countess automated counter. Specimens tzmbl, 293s gnti and 293t17 cell lines listed in various protocols.
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